Figure 5.Dynamic association of SIRT6 and DNA-PKcs with chromatin flanking site-specific DNA DSBs.(a)
SIRT6 occupancy at chromatin flanking DSBs induced by I-PpoI. Quantitative
real-time PCR amplification of DNA sequences flanking the I-PpoI site from
ChIPs performed with SIRT6 antibodies. Data are normalized to no I-PpoI
(control) samples. (b) DNA-PKcs occupancy at chromatin flanking
DSBs induced by I-PpoI, determined as for SIRT6 in (a). (c)
DNA-PKcs occupancy at the indicated distances from an I-SceI DSB site in
SIRT6 KD and controls cells. Data are normalized to no I-SceI controls. (d)
DNA-PKcs occupancy at chromatin adjacent to (+60 to +223 bp) an I-SceI DSB
site, following retroviral over-expression
of Flag-tagged wild-type SIRT6 (S6WT), catalytically inactive SIRT6 (S6HY),
or empty vector control (pBabe). In all panels, SIRT6 KD cells were
generated with S6KD2 shRNA, and the data represent the mean +/- S.E.
