Figure 4.SIRT6 stabilizes DNA-PKcs at chromatin in response to DNA damage.(a)
SIRT6 is required for efficient mobilization of DNA-PKcs to chromatin in
response to the DNA DSB agent NCS. Western analysis with the indicated
antibodies of S6KD2 and control cells fractionated as in (Fig 2c). (b)
Fractionation experiments performed as in (c) utilizing S6KD1 cells
(second independent SIRT6 shRNA). WCE, whole cell extract. Chr,
chromatin. (b) Western analysis showing reconstitution of S6KD2
cells with recombinant wild-type (WT) or catalytically mutant (HY) SIRT6
protein. (d) DNA-PKcs mobilization to chromatin upon NCS treatment
was determined in S6KD2 cell lines reconstituted with WT SIRT6,
catalytically inactive SIRT6 HY protein, or control vector (Co) as
indicated.
