Figure 3.SIRT6 is required for global deacetylation of H3K9 in response to DNA damage.(a) Western analysis of SIRT6 expression levels in the indicated
cell lines stably expressing two different SIRT6 shRNAs (S6KD1 and S6KD2)
or empty vector control (pSR). (b) SIRT6 knock-down leads to
hypersensitivity to γ-irradiation (IR). Gy, Gray. (c)
Western analysis of H3K9Ac levels in S6KD2 (KD) or control (Co) HT1080
cells in response to DNA DSB agents neocarzinostatin (NCS) or bleomycin
(Bleo) treatment (1hr). (d) Western analysis of H3K9Ac levels in
293T cells over-expressing SIRT6 or empty vector control (pcDNA), following
exposure to NCS (1hr). In (c) and (d), total H3 or H3K14Ac levels
are shown as controls.
