Figure 7.Repression of E2F1-regulated genes by Hdm2 or HdmX knockdown is blocked by concurrent knockdown of p21. MCF7 cells were transfected
with the indicated siRNA combinations. Twenty-four hours later, RNA was
isolated and subjected to RT-qPCR to quantify expression of CCNA2, p21 and
E2F1 after normalization to GAPDH. Expression levels (Y-axis) were
relative to siCon and reported as RQ values. Error bars represent the 95%
confidence interval of the relative expression.
