Figure 3.Knockdown of HdmX enhances doxorubicin-induced cytotoxicity.(A) Percent cell viability relative to untransfected untreated
control cells. MCF7 cells were treated with doxorubicin (0.25-1.0
μg/mL) for 48 hours and cell viability was determined by absorbance at
590 nm. The loss of HdmX and/or Hdm2 showed an enhanced cytotoxicity
relative to control cells. (B) RT-qPCR analysis of hdmX, hdm2, p21
and p53 gene expression in the indicated siRNA transfected MCF7 cells. The
hdmX, hdm2, and p53 transcripts were effectively knocked down by siRNA
prior to drug treatment.
