Figure 2.Loss of HdmX and/or Hdm2 inhibits MCF7 colony formation.(A) Following siRNA
transfections, MCF7 cells were seeded at 500 cells/well in 6-well plates.
The cells were allowed to grow for ten days then the colonies were stained
with crystal violet. Significantly fewer colonies were present following
knockdown of HdmX and/or Hdm2. The cells transfected with sip53 or a
non-targeting control (siCon) showed minimal effects on colony formation
relative to non-transfected control (Con/Control). (B) The percent
cell growth relative to untransfected control was determined by extracting
the stain in 10% acetic acid and quantifying the stain by reading
absorbance at 590 nm.
