Figure 1. (A) RT-PCR analysis of hdmX and hdm2 gene expression in various human cell lines. The endogenous levels of hdmX and hdm2 were determined relative to H1299 cells. All samples were
normalized to GAPDH. (B) RNAi knockdown of HdmX or Hdm2 triggers
p53-dependent p21 induction. Western blot analysis of indicated proteins
from the various siRNA or doxorubicin (Dox) treated MCF7 cells. Knockdowns
of the indicated proteins were greater than 80%. Protein extracts were made
24 hours after the last siRNA transfection or treatment with 5 μg/ml doxorubicin.
