Figure 5.Analysis of donor cell engraftment in recipients after BM-INF without prior conditioning. Representative
immunohistochemical analysis of EGFP expression (brown) in ovaries of aged wild-type females following 15 once-monthly
infusions of BM harvested from young adult β-actin-EGFP transgenic female donors (A, B). The ovary of a representative
transgenic donor female (C) is shown as a positive control. (D) Chimerism analysis of BM-derived cells collected from
female mice following 15 once-monthly infusions of β-actin -EGFP transgenic BM (BM-INF, X15) or a single infusion of
β-actin-EGFP transgenic BM (BM-INF, X1). Parallel analysis of BM harvested from recipient females conditioned with
busulfan and cyclophosphamide prior to BMT (again using β transgenic females as donors) is shown for comparison.
For these samples, BM was collected 2 weeks (wk) and 4 weeks post-BMT. The data shown represent the mean ± SEM of results
from analysis of 4 mice per group.
