Research Paper Volume 16, Issue 13 pp 10832—10840
Long non-coding RNA HOTTIP promotes renal cell carcinoma progression through the regulation of the miR-506 pathway
- 1 Department of Thyroid Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China
- 2 Department of Urology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400000, China
Received: October 30, 2023 Accepted: February 8, 2024 Published: June 19, 2024
https://doi.org/10.18632/aging.205947How to Cite
Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
HOXA transcript at the distal tip (HOTTIP), a lncRNA, induces cell proliferation and cancer progression. However, the expression and function of HOTTIP in renal cell carcinoma (RCC) were rarely reported. The role of the HOTTIP in RCC was explored in this study. HOTTIP expresses higher in RCC tissues than in normal tissues and indicates poor prognosis based on the TCGA database. The over- and low-expression HOTTIP cell line was established in this research to assess the oncogenic function of HOTTIP in RCC progression. Mechanistic analyses revealed that HOTTIP functioned as a competing endogenous RNA (ceRNA) for miR-506. RIP experiment and luciferase assay were performed to explore the mechanisms of the sponge between HOTTIP and miR-506. HOTTIP down-regulation attenuated cell proliferation, migration, and invasion, which could be rescued by miR-506 down-regulation. On the whole, this study revealed that the HOTTIP/miR-506 axis has a dominant impact on RCC progression and potentially provides a novel strategy for RCC diagnosis and therapy.