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Research Paper|Volume 16, Issue 9|pp 7902—7914

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules

Wei Li1, Dayong Du1, Yan Huang5, Cui Xu4, Yang Liu1, Xiaohong Wu1, Jie Yang1, Zhipeng Liu4, Jianxin Ma3, Chaoji Huangfu2
  • 1Department of Cardiology, The 305 Hospital of People’s Liberation Army of China, Beijing 100017, China
  • 2Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China
  • 3Department of Cadre Ward, The 305 Hospital of People’s Liberation Army of China, Beijing 100017, China
  • 4Department of Medical Administration, The 305 Hospital of People's Liberation Army of China, Beijing 100017, China
  • 5Department of Neurology, Chengdu Third People’s Hospital, Chengdu 610014, China
* Equal contribution
& This corresponding author has a verified history of publications using a personal email address for correspondence.
Received: January 5, 2024Accepted: April 10, 2024Published: May 3, 2024
https://doi.org/10.18632/aging.205792

Copyright: © 2024 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: Traditional bandages, gauze, and cotton balls are increasingly insufficient for addressing complex war injuries characterized by severe bleeding and diverse wound conditions. The giant salamander, a species of high medical value, secretes a unique mucus when stimulated, which has potential applications in wound care.

Materials: Giant salamander skin mucus gel dressing wrapped with bone marrow mesenchymal stem cells (BMSCs-GSSM-gel) was prepared and validated. Skin wound injury of rabbit and mouse models were established. Hematoxylin and Eosin, Masson's trichrome, and Sirius red staining were performed. The platelet aggregation rate and coagulation items were measured. Transcriptome sequencing was performed to find potential differential expression genes.

Results: Preparation and characterization of BMSCs-GSSM-gel were performed, and BMSCs-GSSM-gel particles with a diameter of about 200 nm were obtained. BMSCs-GSSM-gel accelerated wound healing in both rabbit and mouse models. BMSCs-GSSM-gel significantly promoted hemostasis via increasing platelet aggregation rate and fibrinogen, but decreasing activated partial thromboplastin time, thrombin time, and prothrombin time. BMSCs-GSSM-gel treatment significantly impacted several genes associated with cell adhesion, inflammatory response, collagen-containing extracellular matrix, and the positive regulation of cell migration based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Integrin Subunit Beta 4 (ITGB4), Integrin Subunit Alpha 3 (ITGA3), and Laminin Subunit Beta 3 (LAMB3) might be involved in the wound healing process by BMSCs-GSSM-gel.

Conclusions: We proved the BMSCs-GSSM-gel greatly improved the skin wound healing, and it might play a crucial role in the application fields of skin damage repair.