Research Paper|Volume 15, Issue 18|pp 9426—9437

The mechanism of simultaneous intake of Jujuboside A and B in the regulation of sleep at the hypothalamic level

Wei Wang¹, Yi Wang², Hongyan Pei⁴, Mingming Li¹, Aozhe Zhu¹, Rui Du⁴, Gao Jun Peng³

¹College of Humanities and College of Home Economics, Jilin Agricultural University, Changchun 130118, China
²College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
³New Vita Bioengineering Tianjin Co., Ltd, Tianjin 301726, China
⁴College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China

* Equal contribution

Received: April 21, 2023Accepted: July 6, 2023Published: September 5, 2023

https://doi.org/10.18632/aging.204995

Copyright: © 2023 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

To study the effect of co-administration of Jujuboside A and B (Ju A+B) on sleep, healthy KM mice were given different doses of Ju A+B with a behavioral evaluation of sleep state. Serum levels of key neurotransmitters (5HT, DA, and NE) were measured. The hypothalamus of KM mice was analyzed for differential protein expression using TMT quantitative proteomics, and differential expression protein (DEP) bioinformatics analysis was used to explore potential mechanisms. The result shows that Ju A+B affects sleep by expressing the protein in the hypothalamus. Compared with the control group, the test group showed 10 up-regulated and 139 down-regulated DEPs. The key DEPs were found at the tight junction. Western blot showed reliable alteration of the key DEPs in proteomics. The result of interaction network analysis attributed the potential of Jujuboside to the changes in blood-brain barrier, which provided basic and theoretical data for the efficacy evaluation and mechanism of Jujuboside.