This article has been corrected: The authors corrected Table 3, “Sequence of primers for RT-PCR and long PCR,” because they forgot to update this table before submission. The primers used in the study were changed three times before a set that worked was synthesized and verified by “Sangon Biotech.” They also found and corrected a duplication in Figure 5B created during the figure assembly - column chart “PGC-1α” replicates column chart “Fis1.” Correction was done with data from the original sets of Western blots for PGC-1α protein. These corrections have no impact on the experimental outcome or conclusions.

The corrected Table 3 and Figure 5 are presented below.

Table 3. Sequences of primers for RT-PCR and long PCR.
Target GenePrimer SequenceTm (°C)
OPA1Forward: 5’-TGGTTCGAGAGTCGGTTGAA-3’56
Reverse: 5’- CCTCCCAGTGCTTTGGAGTA -3’56
Mfn1Forward: 5’-GGGAAGACCAAATCGACAGA-3’57
Reverse: 5’-CAAAACAGACAGGCGACAAA-3’57
Mfn2Forward: 5’-GAGAGGCGATTTGAGGAGTG-3’58
Reverse: 5’-CTCTTCCCGCATTTCAAGAC-3’56
Drp1Forward: 5’-GCCCGTGGATGATAAAAGTG-3’56
Reverse: 5’-TGGCGGTCAAGATGTCAATA-3’56
Fis1Forward: 5’-AGATGGACTGGTAGGCATGG-3’56
Reverse: 5’-GACACAGCCAGTCCAATGAG-3’56
PGC-1αForward: 5’-GGACGAATACCGCAGAGAGT-3’59
Reverse: 5’-CCATCATCCCGCAGATTTAC-3’56
TfamForward: 5’-TCACCTCAAGGGAAATTGAAG-3’55
Reverse: 5’-CCCAATCCCAATGACAACTC-3’56
Long FragmentForward:5’-AAAATCCCCGCAAACAATGACCACCC-3’72
Reverse: 5’-GGCAATTAAGAGTGGGATGGAGCCAA-3’72
Short
Fragment		Forward: 5’-CCTCCCATTCATTATCGCCGCCCTGC-3’60
Reverse: 5’-GTCTGGGTCTCCTAGTAGGTCTGGGAA-3’60
BaxForward: 5’-GCGATGAACTGGACAACAAC-3’57
Reverse: 5’-GATCAGCTCGGGCACTTTAG-3’58
Bcl-2Forward: 5’-CGAGTGGGATACTGGAGATGA-3’58
Reverse: 5’- GACGGTAGCGACGAGAGAAG-3’59
Caspase-3Forward: 5’-GACTGGAAAGCCGAAACTCT-3’55
Reverse: 5’-TGCCATATCATCGTCAGTTCC-3’54
Caspase-9Forward: 5’-CAGAGGTTCTCACACCAGAAA-3’54
Reverse: 5’-TGCCATATCTGCATGTCTCTC-3’54
ASK1Forward: 5’-GACAAGAGAGCCTGTGCTAAT-3’54
Reverse: 5’-TCTCCGTGCAACCACATAC-3’55
JNKForward: 5’-GGATTTGGAGGAGCGAACTAA -3’54
Reverse: 5’-CATTGACAGACGGCGAAGA-3’55
Cty-cForward: 5’-GGACAGCCCCGATTTAAGTA-3’57
Reverse: 5’-TCAATAGGTTTGAGGCGACAC-3’58
GAPDHForward: 5’- AGGTCGGTGTGAACGGATTTG -3’58
Reverse: 5’- GGGGTCGTTGATGGCAACA-3’58
Figure 5.
JPYS improved mitochondrial biogenesis and dynamics in premature ovarian failure (POF) rats. Rats were treated with JPYS (11.0 g/kg.d) and pre-treated with triptorelin (1.5 mg/kg) followed by intraperitoneally injected cyclophosphamide (50 mg/kg). We used real-time qPCR and western blot to detect mitochondrial biogenesis and dynamics. We chose OPA1, Mfn1, and Mfn2 to represent mitochondrial biogenesis function, and PGC-1α to represent the dynamic mitochondrial fusion, and Drp1 and Fis1 to represent mitochondrial fission. The expression of OPA1, Mfn1, Mfn2, PGC-1α, Drp1, and Fis1 in mRNA (A) and protein (B) levels. Data are shown as mean ± SD. *p < 0.05 versus control group, #p < 0.05 versus POF group, △p < 0.05 versus JPYS group. (n=6).