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Research Paper|Volume 15, Issue 6|pp 2082—2096

Single cell sequencing analysis constructed the N7-methylguanosine (m7G)-related prognostic signature in uveal melanoma

Jiaheng Xie1, Liang Chen2, Yuan Cao3, Chenfeng Ma4, Wenhu Zhao5, JinJing Li6, Wen Yao3, Yiming Hu7, Ming Wang1, Jingping Shi1
  • 1Department of Burn and Plastic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu, China
  • 2Department of Hepatobiliary Surgery, Jiaxing First Hospital, Jiaxing 314001, Zhejiang, China
  • 3Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China
  • 4Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, Jiangsu, China
  • 5Hepatobiliary/Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China
  • 6Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, Jiangsu, China
  • 7College of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China
* Co-first author
Received: December 10, 2022Accepted: March 6, 2023Published: March 14, 2023
https://doi.org/10.18632/aging.204592

Copyright: © 2023 Xie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: Uveal melanoma is a highly malignant tumor in the eye. Its recurrence and metastasis are common, and the prognosis is poor.

Methods: The transcriptome data of UVM were downloaded from TCGA database, and the single cell sequencing dataset GSE139829 was downloaded from GEO database. Weighted co-expression network analysis was used to explore the modules associated with m7G. Lasso regression was used to construct M7G-related prognostic signature. Immune infiltration analysis was used to explore the significance of the model in the tumor immune microenvironment. Finally, cell assays were used to explore the function of key genes in the MUM-2B and OCM-1 cell lines of UVM.

Results: The prognostic signature was constructed by Cox regression and Lasso regression. Patients could be divided into high-risk group and low-risk group by this signature, and the high-risk group had worse prognosis (P<0.05). Cell experiments showed that the proliferation, invasion and migration ability of UVM cell lines were significantly decreased after the knockdown of PAG1, a key gene in signature, which proved that PAG1 might be a potential target of UVM.

Conclusions: Our study explored the significance of m7G in UVM, provided biomarkers for its diagnosis and treatment.