Research Paper|Volume 15, Issue 5|pp 1524—1542

High expression of TARS is associated with poor prognosis of endometrial cancer

Lihui Si¹, Lianchang Liu², Ruiqi Yang³, Wenxin Li¹, Xiaohong Xu¹

¹Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun 130021, China
²Department of Intervention, The Second Hospital of Jilin University, Changchun 130021, China
³Physical Examination Center, The Second Hospital of Jilin University, Changchun 130021, China

Received: October 24, 2022Accepted: February 20, 2023Published: March 6, 2023

https://doi.org/10.18632/aging.204558

Copyright: © 2023 Si et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Introduction: Endometrial cancer is the second largest and most common cancer in the world. It is urgent to explore novel biomarkers.

Methods: Data were obtained from The Cancer Genome Atlas (TCGA) database. The receiver operating characteristic (ROC) curves, Kaplan-Meier curves and Cox analysis, nomograms, gene set enrichment analysis (GSEA) were conducted. Cell proliferation experiments were performed in Ishikawa cell.

Results: TARS was significantly highly expressed in serous type, G3 grade, and deceased status. Significant association was between high TARS expression with poor overall survival (P = 0.0012) and poor disease specific survival (P = 0.0034). Significant differences were observed in advanced stage, G3 and G4, and old. The stage, diabetes, histologic grade, and TARS expression showed independent prognostic value for overall survival of endometrial cancer. The stage, histologic grade, and TARS expression showed independent prognostic value for disease specific survival of endometrial cancer. Activated CD4⁺ T cell, effector memory CD4⁺ T cell, memory B cell and type 2 T helper cell may participate in the high TARS expression related immune response in endometrial cancer. The CCK-8 results showed significantly inhibited cell proliferation in si-TARS (P < 0.05) and promoted cell proliferation in O-TARS (P < 0.05), confirmed by the colony formation and live/dead staining.

Conclusion: High TARS expression was found in endometrial cancer with prognostic and predictive value. This study will provide new biomarker TARS for diagnosis and prognosis of endometrial cancer.