Research Paper Volume 14, Issue 15 pp 6187—6201
Artemisia argyi exhibits anti-aging effects through decreasing the senescence in aging stem cells
- 1 Department of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan
- 2 Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan
- 3 School of Post-Baccalaureate Chinese Medicine, College of Medicine, Tzu Chi University, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan
- 4 Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan
- 5 Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan
- 6 Department of Sports Sciences, University of Taipei, Taipei 111, Taiwan
- 7 Buddhist Compassion Relief Tzu Chi Foundation, Hualien 970, Taiwan
- 8 Bioinnovation Center, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan
- 9 Department of Neurosurgery, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan
- 10 Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan
- 11 Department of Biotechnology, Bharathiar University, Coimbatore 641 046, India
- 12 Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan
- 13 Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan
- 14 Department of Biotechnology, Asia University, Taichung 41354, Taiwan
- 15 Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien 970, Taiwan
Received: November 11, 2021 Accepted: July 1, 2022 Published: August 9, 2022
https://doi.org/10.18632/aging.204210How to Cite
Copyright: © 2022 Ho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Aging is accompanied by functional loss of many cellular pathways, creating an increased risk of many age-related complications (ARC). Aging causes stem cell exhaustion with a concomitant increase in cellular dysfunction. Recently, interest in senotherapeutics has been growing rapidly to promote healthy aging and as an intervention for ARCs. This research focused on screening the senomorphic properties of Artemisia argyi, as an emerging strategy for longevity, and prevention or treatment of ARCs. In this study, we aimed to find the clinical efficacy of daily consumption of Artemisia argyi water extract (AAW) on aging. In vitro 0.1μM Doxorubicin induced senescent human adipose derived mesenchymal stem cells was treated with different concentrations of AAW to show its anti-aging effect. 15 months old SHR rats (n=6) were treated with 7.9 mg/ml AAW for 4 weeks and anti-aging effect was evaluated. In vitro study showed the protective effect of AAW in telomere shortening and helps in maintaining a balance in the expression of anti-aging protein Klotho and TERT. AAW effectively reduced mitochondrial superoxide and also provided a protective shield against senescence markers like over-expression of p21 and formation of double strand breaks, which is known to cause premature aging. Moreover, animal studies indicated that AAW promoted the expression of Klotho in naturally aging rats. In addition, AAW successfully restored the decline cardiac function and improved the grip strength and memory of aging rat. These findings showed that therapeutic targeting of senescent stem cells by AAW restored stem cell homeostasis and improves overall health.