Research Paper|Volume 13, Issue 24|pp 26022—26033

LINC00839 knockdown restrains the metastatic behavior of nasopharyngeal carcinoma by sponging miR-454-3p

Feng Ying Zhang¹, Xia Li², Ting Ting Huang¹, Mei Ling Xiang¹, Lin Lin Sun¹, Zhao Lan Sun³

¹Department of Otorhinolaryngology, Weifang Hospital of Traditional Chinese Medicine, Weifang, Shandong, China
²Department of Anesthesiology, Weifang Hospital of Traditional Chinese Medicine, Weifang, Shandong, China
³The First Clinical Medical College of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China

* Equal contribution

Received: June 4, 2021Accepted: November 22, 2021Published: December 29, 2021

https://doi.org/10.18632/aging.203792

Copyright: © 2021 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Long intergenic non-coding RNA 00839 (LINC00839) has been verified as a pro-metastasis factor in malignancies. However, the significance of LINC00839 in nasopharyngeal carcinoma (NPC) has yet to be illuminated, as well as its underlying mechanism. Here, we disclosed that LINC00839 is highly expressed in NPC. Deletion of LINC00839 suppresses NPC cells rapid growth, invasive capacity and EMT in vitro. Besides, LINC00839 is identified as a “sponge” for miR-454-3p, and upregulation of LINC00839 reverses miR-454-3p-mediated inhibition of aggressiveness in NPC cells. Furthermore, the expression of cellular-mesenchymal epithelial transition factor (c-Met), the downstream target of miR-454-3p, is downregulated by LINC00839 knockdown in NPC cells. In vivo, LINC00839 knockdown retards the tumor growth of NPC cells in the xenografted mice model. Collectively, attenuation of LINC00839 expression attenuates the aggressive properties of NPC cells via directly sponging the miR-454-3p and regulating c-Met expression.