Research Paper Volume 13, Issue 20 pp 23588—23602
ALDH2 promotes uterine corpus endometrial carcinoma proliferation and construction of clinical survival prognostic model
- 1 School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, Shandong, PR China
- 2 Department of Medical Laboratory, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, Hubei, PR China
- 3 Institute of Biology and Medicine, College of Life and Health Sciences, Wuhan University of Science and Technology, Hubei 430081, PR China
- 4 Hubei Province Hospital of Traditional Chinese and Western Medicine, Hubei 430010, PR China
Received: July 23, 2021 Accepted: September 20, 2021 Published: October 20, 2021
https://doi.org/10.18632/aging.203605How to Cite
Copyright: © 2021 Cui et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
UCEC is one of the three common malignant tumors of the female reproductive tract. According to reports, the cure rate of early UCEC can reach 95%. Therefore, the development of prognostic markers will help UCEC patients to find the disease earlier and develop treatment earlier. The ALDH family was first discovered to be the essential gene of the ethanol metabolism pathway in the body. Recent studies have shown that ALDH can participate in the regulation of cancer. In our research, we explored the expression of the ALDH family in 33 cancers. It was found that ALDH2 was abnormally expressed in UCEC. Besides, in vivo and in vitro experiments were conducted to explore the effect of ALDH2 expression on the proliferation of UCEC cell lines. Meanwhile, the change of its expression is not due to gene mutations, but is regulated by miR-135-3p. At the same time, the impact of ALDH2 changes on the survival of UCEC patients is deeply discussed. Finally, a nomogram for predicting survival was constructed, with a C-index of 0.798 and AUC of 0.764. This study suggests that ALDH2 may play a crucial role in UCEC progression and has the potential as a prognostic biomarker of UCEC.
Abbreviations
UCEC: Uterine Corpus Endometrial Carcinoma; TCGA: The Cancer Genome Atlas; OS: Overall Survival; dfI: Disease Free Survival; Dss: Disease Free Survival; PFI: Progression Free Survival; DEGs: Differentially Expressed Genes; qRT-PCR: Real-time PCR; PPI: Protein Protein Interaction Network; WB: Western Blot; NC: Negative Control.