Research Paper|Volume 13, Issue 10|pp 14342—14354

LncRNA MEG8 promotes TNF-α expression by sponging miR-454-3p in bone-invasive pituitary adenomas

Hai-Bo Zhu², Bin Li¹, Jing Guo¹, Ya-Zhou Miao¹, Yu-Tao Shen¹, Ya-Zhuo Zhang^1,^2,^3,⁴, Peng Zhao², Chu-Zhong Li^1,^2,^3,⁴

¹Beijing Neurosurgical Institute, Capital Medical University, Fengtai 100070, Beijing, China
²Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Fengtai 100070, Beijing, China
³Beijing Institute for Brain Disorders Brain Tumor Center, Fengtai 100070, Beijing, China
⁴China National Clinical Research Center for Neurological Diseases, Fengtai 100070, Beijing, China

* Equal contribution

Received: May 23, 2020Accepted: February 16, 2021Published: May 19, 2021

https://doi.org/10.18632/aging.203048

Copyright: © 2021 Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

There are few studies on the mechanism of pituitary adenoma (PA) destroying bone. The current study aimed to investigate the role of MEG8/miR-454-3p/TNF-α in bone-invasive pituitary adenomas (BIPAs). In this study, we report that lncRNA MEG8 and TNF-α are upregulated in BIPA tissues while miR-454-3p is downregulated, which is associated with poor progression-free survival (PFS). Functional assays revealed the role of up-regulated MEG8 and down-regulated miR-454-3p in promoting bone destruction. Mechanistically, MEG8 promotes TNF-α expression by sponging miR-454-3p, which ultimately leads to the occurrence of bone destruction. The mechanism is confirmed in vivo and in vitro. Therefore, our data illustrated a new regulatory mechanism of MEG8/miR-454-3p/TNF-α in BIPAs. It may provide a useful strategy for diagnosis and treatment for BIPA patients.