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Research Paper|Volume 13, Issue 8|pp 11808—11821

Exosomes derived from miR-1228 overexpressing bone marrow-mesenchymal stem cells promote growth of gastric cancer cells

Lili Chang1, He Gao2, Li Wang1, Ning Wang1, Shumei Zhang1, Xiaona Zhou1, Huijun Yang3
  • 1Department of Gastroenterology, The First Ward, Shijiazhuang First Hospital, Shijiazhuang, Hebei Province, China
  • 2Department of General Practice, Shijiazhuang First Hospital, Shijiazhuang, Hebei Province, China
  • 3Department of Infectious Disease, Shijiazhuang First Hospital, Shijiazhuang, Hebei Province, China
* Equal contribution
Received: July 10, 2020Accepted: October 27, 2020Published: April 21, 2021
https://doi.org/10.18632/aging.202878

Copyright: © 2021 Chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

There has been increasing evidence that microRNAs (miRNAs) are related to glioma progression, and that genetically engineered mesenchymal stem cells (MSCs) can inhibit the growth of gliomas. However, the underlying mechanism of bone marrow-MSCs (BM--MSCs) and miRs in gastric cancer still remains unclear. Patients with gastric cancer treated in Shijiazhuang First Hospital as well as healthy individuals undergoing physical examinations were recruited to measure the expression of exosomal miR-1228. Receiver operating characteristic (ROC) curves were plotted and the patients were followed up. BM--MSCs from healthy subjects were collected and exosomes were extracted. The MSC cells were transfected with lentiviral vectors carrying miR-1228 and MMP-14 over-expression sequences and scramble sequence, followed by exosome extraction. The exosomes were co-cultured with SGC-7901 and MGC-823 cells to detect cell proliferation, invasion, apoptosis and migration. The correlation between miR-1228 and MMP-14 was determined by dual-luciferase reporter assay. miR-1228 was highly expressed in serum exosomes of patients with gastric cancer with a area under ROC curve (AUC) of 0.865. The exosomes derived from BM-MSCs are expected to be efficient nanocarriers. Up-regulation of miR-1228 can down-regulate the expression of MMP-14 and effectively hinders the development and progression of gastric cancer.