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Research Paper|Volume 13, Issue 2|pp 2593—2603

Antitumor effect of poly lactic acid nanoparticles loaded with cisplatin and chloroquine on the oral squamous cell carcinoma

Qiang Li1, Xia Liu2, Wei Yan1, Yong Chen1
  • 1Department of Oral and Maxillofacial Surgery, Cangzhou Central Hospital, Cangzhou, Hebei Province, China
  • 2Department of Basic Nursing, Cangzhou Medical College, Cangzhou, Hebei Province, China
Received: February 8, 2020Accepted: April 17, 2020Published: December 11, 2020
https://doi.org/10.18632/aging.202297

Copyright: © 2020 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Purpose: Poly lactic acid (PLA) combined with cisplatin-chloroquine nanoparticles (CDDP/CQ-PLA NPs) and PLA combined with cisplatin nanoparticles (CDDP-PLA NPs) were prepared to investigate their inhibitory effects on the proliferation of oral squamous cell carcinoma (OSCC) Cal-27cell line.

Patients and methods: We prepared CDDP/CQ-PLA NPs and CDDP-PLA NPs. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) were used to detect the physiological characteristics and particle size parameters of drug-loaded nanoparticles. The drug concentration and cumulative release were measured by UV and visible spectrophotometer. MTT assay was used to detect viability of Cal-27 cells. Annexin/PI staining was used to detect cell apoptosis. Biological kits were used to detect malondialdehyde (MDA) content, catalase (CAT) activity, antioxidant enzyme superoxide dismutase (SOD) activity and glutathione peroxidase (GSH PX) activity in Cal-27 cells. Western blot was used to detect apoptosis and autophagy of Cal-27 cells.

Results: CDDP/CQ-PLA NPs and CDDP -PLA NPs had good drug loaded nanoparticles and drug release. CDDP/CQ-PLA NPs showed higher ROS and apoptosis rate, and lower autophagy than CDDP-PLA NPs.

Conclusion: CDDP/CQ-PLA NPs reduced autophagy and enhanced ROS and apoptosis of Cal-27 cells, which shows a potential in the clinical treatment of OSCC.