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Research Paper Volume 12, Issue 14 pp 14775—14790
The circular RNA circEIF3M promotes breast cancer progression by promoting cyclin D1 expression
- 1 Department of General Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China
- 2 Department of Pathology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China
- 3 Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China
Received: January 1, 2020 Accepted: May 20, 2020 Published: July 11, 2020
https://doi.org/10.18632/aging.103539How to Cite
Abstract
We investigated the function of circular RNA circEIF3M (hsa_circ_0003119) in triple-negative breast cancer. The expression profiles of circRNAs in 3 specimens of triple-negative breast cancer tissues with adjacent nontumor tissues were analyzed by RNA-sequencing. We verified the oncogenic role of circEIF3M in triple-negative breast cancer through a series of biological function experiments. It was found that circEIF3M was markedly upregulated in triple-negative breast cancer as compared to adjacent nontumor tissue, and that circEIF3M promoted triple-negative breast cancer cell proliferation, migration, and invasion. Mechanistic analysis indicated that circEIF3M may act as a competing endogenous RNA for miR-33a that relieves the inhibitory effect of miR-33a on its target cyclin D1. These findings showed that circEIF3M promotes triple-negative breast cancer progression via the circEIF3M/ miR-33a/ cyclin D1 axis.
Abbreviations
circRNAs: circular RNAs; circRNA: circular RNA; TNBC: triple-negative breast cancer; ER: estrogen receptor; PR: progesterone receptor; HER2: human epidermal growth factor receptor-2; ceRNA: competing endogenous RNA; miRNA: microRNA; miRNAs: microRNAs; CDR1as: cerebellar degeneration associated protein 1 antisense transcript; RNA-Seq: RNA sequencing; EIF3M: eukaryotic initiation factor 3M; CCND1: cyclin D1; OD: optical density; qRT-PCR: quantitative real time polymerase chain reaction; FITC: fluorescein isothiocyanate; PI: propidium iodide; MRE: miRNA response element; 3’UTR: 3'-untranslated region; WT: wild type; FISH: fluorescence in situ hybridization; CCK8: Cell Counting Kit-8; CDK4: cyclin-dependent kinase 4.