Research Paper|Volume 12, Issue 13|pp 12896—12920

A novel molecular-clinicopathologic nomogram to improve prognosis prediction of hepatocellular carcinoma

Zhongjing Zhang¹, Wanqing Weng¹, Weiguo Huang¹, Boda Wu¹, Yi Zhou¹, Jie Zhang¹, Tuo Deng¹, Wen Ye¹, Jiecheng Zhang¹, Jianyang Ao¹, Qiyu Zhang¹, Keqing Shi²

¹Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325015, Zhejiang Province, PR China
²Precision Medical Center Laboratory, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325015, Zhejiang Province, PR China

* Co-first author

Received: October 4, 2019Accepted: May 20, 2020Published: June 30, 2020

https://doi.org/10.18632/aging.103350

Copyright © 2020 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: Emerging evidence suggests that long non-coding RNA (lncRNA) plays a crucial part in the development and progress of hepatocellular carcinoma (HCC). The objective was to develop novel molecular-clinicopathological prediction methods for overall survival (OS) and recurrence of HCC.

Results: An 8-lncRNA-based classifier for OS and a 14-lncRNA-based classifier for recurrence were developed by LASSO COX regression analysis, both of which had high accuracy. The tdROC of OS-nomogram and recurrence-nomogram indicates the satisfactory accuracy and predictive power. The classifiers and nomograms for predicting OS and recurrence of HCC were validated in the Test and GEO cohorts.

Conclusions: These two lncRNA-based classifiers could be independent prognostic factors for OS and recurrence. The molecule-clinicopathological nomograms based on the classifiers could increase the prognostic value.

Methods: HCC lncRNA expression profiles from the cancer genome atlas (TCGA) were randomly divided into 1:1 training and test cohorts. Based on least absolute shrinkage and selection operator method (LASSO) COX regression model, lncRNA-based classifiers were established to predict OS and recurrence, respectively. OS-nomogram and recurrence-nomogram were developed by combining lncRNA-based classifiers and clinicopathological characterization to predict OS and recurrence, respectively. The prognostic value was accessed by the time-dependent receiver operating characteristic (tdROC) and the concordance index (C-index).