Research Paper Volume 12, Issue 3 pp 2610—2625
Testicular biopsies microarray analysis reveals circRNAs are involved in the pathogenesis of non-obstructive azoospermia
- 1 Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China
- 2 Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China
- 3 Hunan Cancer Hospital and The Affliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China
Received: September 11, 2019 Accepted: January 12, 2020 Published: February 6, 2020
https://doi.org/10.18632/aging.102765How to Cite
Abstract
Circular RNAs (circRNAs) have been reported to be involved in many diseases. But there is no report on circRNAs in non-obstructive azoospermia (NOA). The purpose of this paper is to explore the circular RNA expression profile and potential functions of circRNAs in NOA patients. We first preformed circRNA expression profiling analysis using a circRNA microarray in testicular samples from NOA and obstructive azoospermia (OA) patients. CircRNAs were validated by qRT-PCR. Bioinformatics analysis were used to construct the ceRNA network. GO and KEGG enrichment analysis were performed by using DAVID. Microarray analysis identified 82 differentially expressed circRNAs in NOA specimens. The differential expression of hsa_circRNA_402130, hsa_circRNA_072697, hsa_circRNA_030050, hsa_circRNA_100812 and hsa_circRNA_406168 was confirmed by qRT-PCR. Enrichment analysis revealed the association of hsa_circRNA_402130 and hsa_circRNA_072697 with multiple signaling pathways. The data indicated that circRNAs were significantly dysregulated in NOA specimens and might involve in the pathogenesis of NOA.
Abbreviations
ART: assisted reproductive technology; BP: biological processce; ceRNA: competing endogenous RNA; circRNAs: Circular RNAs; FSH: follicle-stimulating hormone; GWAS: genome-wide association analysis; LH: luteinizing hormone; MREs: microRNA response elements; NOA: non-obstructive azoospermia; OA: obstructive azoospermia; qRT-PCR: quantitative real-time reverse transcription PCR; T: testosterone.