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Research Paper|Volume 12, Issue 3|pp 2347—2372

Protein disulfide isomerases are promising targets for predicting the survival and tumor progression in glioma patients

Zhigang Peng1, Yu Chen2, Hui Cao3, Hecun Zou4, Xin Wan1, Wenjing Zeng4, Yanling Liu4, Jiaqing Hu5, Nan Zhang6, Zhiwei Xia7, Zhixiong Liu1, Quan Cheng1,4,8
  • 1Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P. R. China
  • 2Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, Henan, P. R. China
  • 3Department of Psychiatry, The Second People's Hospital of Hunan Province, The Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan, P. R. China
  • 4Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P. R. China
  • 5Department of Emergency, The Second People’s Hospital of Hunan Province, The Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan, P. R. China
  • 6School of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, Heilongjiang, P. R. China
  • 7Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P. R. China
  • 8Institute of Clinical Pharmacology, Central South University; Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, Hunan, P. R. China
* Equal contribution
Received: September 13, 2019Accepted: January 7, 2020Published: February 5, 2020

Copyright: © 2020 Peng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The present study focused on the expression patterns, prognostic values and potential mechanism of the PDI family in gliomas. Most PDI family members’ mRNA expressions were observed significantly different between gliomas classified by clinical features. Construction of the PDI signature, cluster and risk score models of glioma was done using GSVA, consensus clustering analysis, and LASSO Cox regression analysis respectively. High values of PDI signature/ risk score and cluster 1 in gliomas were associated with malignant clinicopathological characteristics and poor prognosis. Analysis of the distinctive genomic alterations in gliomas revealed that many cases having high PDI signature and risk score were associated with genomic aberrations of driver oncogenes. GSVA analysis showed that PDI family was involved in many signaling pathways in ERAD, apoptosis, and MHC class I among many more. Prognostic nomogram revealed that the risk score was a good prognosis indicator for gliomas. The qRT-PCR and immunohistochemistry confirmed that P4HB, PDIA4 and PDIA5 were overexpressed in gliomas. In summary, this research highlighted the clinical importance of PDI family in tumorigenesis and progression in gliomas.