Research Paper Volume 12, Issue 2 pp 1186—1200
Fibroblast growth factor 10 alleviates particulate matter-induced lung injury by inhibiting the HMGB1-TLR4 pathway
- 1 Department of Pulmonary Medicine, Wenzhou Medical University First Affiliated Hospital, Wenzhou 325006, China
- 2 Department of Pulmonary Medicine, Yiwu Central Hospital, Yiwu 322000, China
- 3 Department of Pharmacy, Wenzhou Medical University Pharmacy School, Wenzhou 325006, China
Received: October 2, 2019 Accepted: December 25, 2019 Published: January 20, 2020
https://doi.org/10.18632/aging.102676How to Cite
Abstract
Exposure to particulate matter (PM) is associated with increased incidence of respiratory diseases. The present study aimed to investigate the roles of fibroblast growth factor 10 (FGF10) in PM-induced lung injury. Mice were intratracheally instilled with FGF10 or phosphate-buffered saline at one hour before instillation of PM for two consecutive days. In addition, the anti-inflammatory impact of FGF10 in vitro and its effect on the high-mobility group box 1 (HMGB1)-toll-like receptor 4 (TLR4) pathway was investigated. It was found that PM exposure is associated with increased inflammatory cell infiltration into the lung and increased vascular protein leakage, while FGF10 pretreatment attenuated both of these effects. FGF10 also decreased the PM-induced expression of interleukin (IL)-6, IL-8, tumor necrosis factor-α and HMGB1 in murine bronchoalveolar lavage fluid and in the supernatants of human bronchial epithelial cells exposed to PM. FGF10 exerted anti-inflammatory and cytoprotective effects by inhibiting the HMGB1-TLR4 pathway. These results indicate that FGF10 may have therapeutic values for PM-induced lung injury.
Abbreviations
PM: Particulate matter; FGF10: Fibroblast Growth Factor 10; IL-6: Interleukin-6; IL-8: Interleukin-8; TNF-α: Tumor necrosis factor-α; BALF: Bronchoalveolar lavage fluid; HMGB1: High mobility group box 1 protein; TLR4: Toll-like receptor 4; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; PBS: Phosphate buffered saline; RPMI-1640: Roswell Park Memorial Institute 1640; qRT-PCR: Quantitative reverse transcriptase-polymerase chain reaction.