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Research Paper|Volume 12, Issue 1|pp 902—911

SAG expression associates with COPB2-related signaling and a poorer prognosis in breast cancer

Aiyu Liu1, Shizhen Zhang2, Weihan Li3, Biao Xu4, Rui Lei5, Shengmei Zhu1
  • 1Department of Anesthesiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang, China
  • 2Institute of Translational Medicine, Zhejiang University, School of Medicine, Hangzhou 310029, Zhejiang, China
  • 3Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310005, Zhejiang, China
  • 4Departmant of Galactophore Surgery, The Second Affiliated Hospital of Guangzhou University of traditional Chinese Medicine, Guangdong 510120, China
  • 5Departmant of Plastic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, ZhejiangChina
* Equal contribution
Received: October 17, 2019Accepted: December 24, 2019Published: January 11, 2020
https://doi.org/10.18632/aging.102663

Copyright: © 2020 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

SAG is an essential RING component of the Cullin-RING ligase (CRL) E3 ubiquitin ligase complex, which regulates diverse signaling pathways and biological processes, including cell apoptosis, embryonic development, angiogenesis, and tumorigenesis. In the present study, we revealed that SAG gene expression is upregulated in breast cancer cells and that SAG overexpression is associated with significant poorer survival in breast cancer, especially the luminal A subtype. We also detected highly correlated co-overexpression of SAG and COPB2 in breast cancers. Subsequent in vitro experiments demonstrated that SAG and COPB2 act cooperatively to stimulate breast cancer cell proliferation, migration and invasion. Our findings suggest that levels of SAG and COPB2 expression may be useful prognostic indicators in breast cancers and that SAG may be involved in COPB2-related signaling during breast cancer development.