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Research Paper|Volume 11, Issue 23|pp 11463—11473

LncRNA EPIC1 downregulation mediates hydrogen peroxide-induced neuronal cell injury

Jian Sun1, Jinyu Zheng2, Ya Li3, Ming Yan1, Ping Li4, Lili Ma5
  • 1Department of Anesthesiology, Huai’an Maternity and Child Healthcare Hospital, Yangzhou University Medical School, Huai’an, China
  • 2Department of Neurosurgery, The Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, China
  • 3The Central Lab, North District, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, China
  • 4Department of Radiotherapy and Oncology, Affiliated Kunshan Hospital to Jiangsu University, Kunshan, China
  • 5Department of Anesthesiology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer of Hospital Nanjing Medical University, Nanjing, China
* Equal contribution
Received: September 7, 2019Accepted: November 19, 2019Published: December 8, 2019
https://doi.org/10.18632/aging.102545

Copyright © 2019 Sun et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Excessive oxidative stress causes neuronal cell injury. Long non-coding RNA (LncRNA) EPIC1 (Lnc-EPIC1) is a MYC-interacting LncRNA. Its expression and potential functions in hydrogen peroxide (H2O2)-stimulated neuronal cells are studied. In SH-SY5Y neuronal cells and primary human neuron cultures, H2O2 downregulated Lnc-EPIC1 and key MYC targets (Cyclin A1, CDC20 and CDC45). Ectopic overexpression of Lnc-EPIC1 increased expression of MYC targets and significantly attenuated H2O2-induced neuronal cell death and apoptosis. Contrarily, Lnc-EPIC1 siRNA potentiated neuronal cell death by H2O2. MYC knockout by CRISPR/Cas9 method also facilitated H2O2-induced SH-SY5Y cell death. Significantly, MYC knockout abolished Lnc-EPIC1-induced actions in H2O2-stimulated neuronal cells. Together, these results suggest that Lnc-EPIC1 downregulation mediates H2O2-induced neuronal cell death.