Research Paper|Volume 11, Issue 6|pp 1821—1831

Reactivation of microRNA-506 inhibits gastric carcinoma cell metastasis through ZEB2

Gui-Jun Wang¹, Bao-Ping Jiao¹, Yang-Jun Liu¹, Yan-Rong Li², Bei-Bei Deng³

¹, Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
², Department of Gastroenterology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
³, Department of Clinical Laboratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, China

Received: January 27, 2019Accepted: March 10, 2019Published: March 28, 2019

Copyright: © 2019 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

MicroRNAs (miRNAs) are frequently dysregulated in a variety of human cancers, including gastric carcinoma. To improve our understanding of the role of miRNAs in gastric carcinoma and potential identify novel biomarkers or therapeutic agents, we performed microarray analysis to identify differentially expressed miRNAs in gastric carcinoma, compared with paired non-cancerous gastric tissues. We identified significantly differentially expressed miRNAs in gastric carcinoma tissues, including miR-506. We validated the microarray results by quantitative reverse transcription polymerase chain reaction in 26 specimens and confirmed significant downregulation of miR-506 in gastric carcinoma. Bioinformatics analysis predicted ZEB2 (zinc finger E-box-binding homeobox 2) as a potential target of miR-506. MiR-506 levels and ZEB2 levels were inversely correlated in gastric carcinoma, and low miR-506 levels in gastric carcinoma were associated with poor prognosis. Overexpression of miR-506 in gastric carcinoma cells significantly inhibited cell migration and invasion, while depletion of miR-506 in gastric carcinoma cells significantly increased cell migration and invasion. Transplantation of miR-506-overexpressing gastric carcinoma cells developed significantly smaller tumor, compared to the control. Thus, our results suggest that miR-506 may function as a tumor suppressor and targets and inhibits ZEB2 in gastric carcinoma.