Research Paper|Volume 11, Issue 1|pp 18—32

MicroR-542-3p can mediate ILK and further inhibit cell proliferation, migration and invasion in osteosarcoma cells

Wei Cai¹, Yong Xu¹, Wenshan Zuo¹, Zhen Su²

¹Department of Orthopedics, Huai’An First People’s Hospital, Nanjing Medical University, Huai’an 223300, Jiangsu, China
²Department of Anesthesiology, Huai’An First People’s Hospital, Nanjing Medical University, Huai’an 223300, Jiangsu, China

Received: July 22, 2018Accepted: December 3, 2018Published: January 11, 2019

Copyright: © 2019 Cai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

MiR-542-3p and its target gene integrin linked kinase (ILK) in human osteosarcoma together with the differentially expressed genes from osteosarcoma tissues was analyzed through bioinformatics analysis in this study. Real time quantitative polymerase chain reaction (qRT-PCR) and western blot showed that the miR-542-3p expression decreased while the ILK expression increased in the osteosarcoma tissues. The overexpressed miR-542-3p or silenced ILK restrained cell invasion, proliferation and migration and arrested cell cycle, facilitated cell apoptosis in U-2OS and 143B cells. The dual-luciferase assay confirmed the targeting relationship between miR-542-3p and ILK. MiR-542-3p overexpression inhibited osteosarcoma growth in vivo. In conclusion, miR-542-3p overexpression down-regulated its target gene ILK, promoted osteosarcoma cells apoptosis and inhibited their proliferation, migration and invasion.