Research Paper Volume 10, Issue 6 pp 1390—1401
Five ETS family members, ELF-1, ETV-4, ETV-3L, ETS-1, and ETS-2 upregulate human leukocyte-associated immunoglobulin-like receptor-1 gene basic promoter activity
- 1 Department of Immunology, School of Basic Medical Sciences, Binzhou Medical University, Shandong 264003, China
- 2 Anti-aging Research Institution, Binzhou Medical University, Shandong 264003, China
- 3 School of Agriculture, Ludong University, Shandong 264003, China
- 4 Beijing Key Laboratory of Drug Target and Screening Research, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- 5 School of Gerontology, Binzhou Medical University, Shandong 264003, China
- 6 The People's Liberation Army 107 Hospital, Affiliated Hospital of Bin Zhou Medical University, Yantai 264002, China
- 7 Department of Microbiology, School of Basic Medical Sciences, Binzhou Medical University, Shandong 264003, China
- 8 School of Medicine, University of California - San Diego, La Jolla, CA 92037, USA
Received: December 25, 2017 Accepted: June 10, 2018 Published: June 18, 2018
https://doi.org/10.18632/aging.101475How to Cite
Abstract
Human leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1), an immunoinhibitory receptor, is expressed on most types of hematopoietic cells and some tumor cells. LAIR-1 plays an inhibitory role in immune cell maturation, differentiation, and activation. LAIR-1 is also involved in some autoimmune diseases and tumors. However, the mechanism controlling the regulation of the LAIR-1 gene is still unknown. In order to elucidate the molecular mechanisms involved in LAIR-1 regulation, in the present study, we cloned and characterized the promoter region of LAIR-1 gene using a series of truncated promoter plasmids in luciferase reporter assays. Our results show that the basic core promoter of LAIR-1 is located within the region -256/-8 relative to the translational start site. Our further studies indicate that five ETS transcription factors: ELF-1, ETV-4, ETV-3L, ETS-1 and ETS-2, can up-regulate the LAIR-1 basic promoter activity. Of these, ETS-2 is the most effective transcription factor. Moreover, ETS-2 was confirmed to interact directly with the basic promoter of LAIR-1. This study presents the first description of regions/factors capable of up-regulation the promoter activity of LAIR-1. This new knowledge contributes to understanding of the molecular mechanisms involved in LAIR-1 associated immune regulation and diseases.
Abbreviations
LAIR-1: leukocyte-associated immunoglobulin-like receptor-1; ITIMs: immune receptor tyrosine-based inhibitory motifs; TSS: translational start site; CHIP: Chromatin Immunoprecipitation; EMSA: Electrophoretic mobility shift assays.