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Research Paper|Volume 9, Issue 2|pp 447—474

Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans

Qin-Li Wan1,2,3, Xiaohuo Shi1,3, Jiangxin Liu1,3, Ai-Jun Ding1,2,3, Yuan-Zhu Pu1,2,3, Zhigang Li4, Gui-Sheng Wu1,3,5, Huai-Rong Luo1,3,5
  • 1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
  • 2University of Chinese Academy of Sciences, Beijing 100039, China
  • 3Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming, Yunnan 650201, China
  • 4Shineway Pharmaceutical Co., Ltd, Sanhe, Hebei 065201, China
  • 5Key Laboratory for Aging and Regenerative Medicine, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China
Received: November 27, 2016Accepted: January 31, 2017Published: February 6, 2017
https://doi.org/10.18632/aging.101170

Copyright: © 2017 Wan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In Caenorhabditis elegans (C. elegans), ablation of germline stem cells (GSCs) leads to infertility, which extends lifespan. It has been reported that aging and reproduction are both inextricably associated with metabolism. However, few studies have investigated the roles of polar small molecules metabolism in regulating longevity by reproduction. In this work, we combined the nuclear magnetic resonance (NMR) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) to profile the water-soluble metabolome in C. elegans. Comparing the metabolic fingerprint between two physiological ages among different mutants, our results demonstrate that aging is characterized by metabolome remodeling and metabolic decline. In addition, by analyzing the metabolic profiles of long-lived germline-less glp-1 mutants, we discovered that glp-1 mutants regulate the levels of many age-variant metabolites to attenuate aging, including elevated concentrations of the pyrimidine and purine metabolism intermediates and decreased concentrations of the citric acid cycle intermediates. Interestingly, by analyzing the metabolome of daf-16;glp-1 double mutants, our results revealed that some metabolic exchange contributing to germline-mediated longevity was mediated by transcription factor FOXO/DAF-16, including pyrimidine metabolism and the TCA cycle. Based on a comprehensive metabolic analysis, we provide novel insight into the relationship between longevity and metabolism regulated by germline signals in C. elegans.