Research Paper Volume 8, Issue 11 pp 2871—2896
Age-associated NF-κB signaling in myofibers alters the satellite cell niche and re-strains muscle stem cell function
- 1 Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Cambridge, MA 02138, USA
- 2 Joslin Diabetes Center, Boston, MA 02215, USA
- 3 Division of Plastic Surgery, Brigham and Women’s Hospital, Boston, MA 02115, USA
- 4 Department of Biostatistics, Harvard School of Public Health, MA 02115, USA
- 5 Department of Biomedical Engineering, Boston University, Boston 02215, USA
- 6 Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
- 7 Paul F. Glenn Center for the Biology of Aging, Harvard Medical School, Boston, MA 02115, USA
Received: May 24, 2016 Accepted: October 25, 2016 Published: November 14, 2016
https://doi.org/10.18632/aging.101098How to Cite
Abstract
Skeletal muscle is a highly regenerative tissue, but muscle repair potential is increasingly compromised with advancing age. In this study, we demonstrate that increased NF-κB activity in aged muscle fibers contributes to diminished myogenic potential of their associated satellite cells. We further examine the impact of genetic modulation of NF-κB signaling in muscle satellite cells or myofibers on recovery after damage. These studies reveal that NF-κB activity in differentiated myofibers is sufficient to drive dysfunction of muscle regenerative cells via cell-non-autonomous mechanisms. Inhibition of NF-κB, or its downstream target Phospholipase A2, in myofibers rescued muscle regenerative potential in aged muscle. Moreover, systemic administration of sodium salicylate, an FDA-approved NF-κB inhibitor, decreased inflammatory gene expression and improved repair in aged muscle. Together, these studies identify a unique NF-κB regulated, non-cell autonomous mechanism by which stem cell function is linked to lipid signaling and homeostasis, and provide important new targets to stimulate muscle repair in aged individuals.