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Research Paper|Volume 5, Issue 5|pp 357—372

hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding

Hanadie Yousef2, Michael J. Conboy1, Ju Li1, Matthew Zeiderman2, Tandis Vazin1,3, Christina Schlesinger1, David V. Schaffer1,3, Irina M. Conboy1
  • 1Department of Bioengineering and California Institute for Quantitative Biosciences (QB3), UC Berkeley, Berkeley, CA 94720, USA
  • 2Department of Molecular and Cellular Biology, UC Berkeley, Berkeley, CA 94720, USA
  • 3Department of Chemical Engineering and Helen Wills Neuroscience Institute, UC Berkeley, Berkeley, CA 94720 USA
Received: May 13, 2013Accepted: May 21, 2013Published: May 23, 2013
https://doi.org/10.18632/aging.100559

Copyright: © 2013 Yousef et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

This work builds upon our findings that proteins secreted by hESCs exhibit pro-regenerative activity, and determines that hESC-conditioned medium robustly enhances the proliferation of both muscle and neural progenitor cells. Importantly, this work establishes that it is the proteins that bind heparin which are responsible for the pro-myogenic effects of hESC-conditioned medium, and indicates that this strategy is suitable for enriching the potentially therapeutic factors. Additionally, this work shows that hESC-secreted proteins act independently of the mitogen FGF-2, and suggests that FGF-2 is unlikely to be a pro-aging molecule in the physiological decline of old muscle repair. Moreover, hESC-secreted factors improve the viability of human cortical neurons in an Alzheimer's disease (AD) model, suggesting that these factors can enhance the maintenance and regeneration of multiple tissues in the aging body.