Figure 4.ATF5 is necessary for the normal induction of related ISR/UPRmt transcription factors following acute contractile activity with age. (A) Representative immunoblots of Total eIF2α, Phosphorylated eIF2α, Total JNK2, Phosphorylated JNK2, ATF4 and CHOP corrected to Ponceau, with respective quantifications (B–E). The approximate molecular weights of proteins are indicated with a hash bar in kilodaltons (kDa). (n=4-7/group). S† P<0.05, S†† P<0.01, S††† P<0.001, S†††† P<0.0001, main effect of acute contractile stimulation; A†† P<0.01, A††† P<0.001, main effect of age; G††† P<0.001, main effect of genotype; AxG† P<0.05, AxG†† P<0.01, interaction between age and genotype. AxGxS† P<0.05, interaction between age, genotype and stimulation; Three-way ANOVA. Y, Young; A, Aged; Con, Control; Stim, acute in situ stimulation. Data are means ± SD.
