Research Paper Advance Articles

Figure 1. The SAAR diet and BSO induce similar morphometric changes. Four groups of eighteen-week-old male C57BL/6NTac mice (n=7/group) were fed high-fat (60% Kcal from fat) diets with 0.86% w/w methionine (CD), 0.12% w/w methionine (SAAR), SAAR diet with 30 mM N-acetylcysteine in water (NAC), and CD diet with 30 mM DL-buthionine (S, R) sulfoximine (BSO). Compared to the CD, the SAAR diet caused weight loss (A–C) and increased food intake (D). Despite feeding on the SAAR diet, mice in the NAC group did not exhibit any changes (A–D). Except for the lack of increased food intake, changes in BSO mice were similar to those in the SAAR mice, despite feeding on a diet replete with sulfur amino acids (A–D). Note: One-way ANOVA followed by Tukey’s multiple comparison tests was used to find group-wise differences (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Bars and error bars represent means and standard error of means). CD growth curves are invisible as NAC growth curves overlay them.