Research Paper Volume 17, Issue 3 pp 822—850

Differential senolytic inhibition of normal versus Aβ-associated cholinesterases: implications in aging and Alzheimer’s disease

Figure 3. Lineweaver-Burk (LB) plots showing inhibition of butyrylcholinesterase (BChE) upon treatment with varying concentrations of senolytic and nootropic agents 1, 3, 4 and 6 (A–D, respectively). Compounds 2 and 5 showed too little inhibition to complete LB kinetics. Each compound was assessed at a range of concentrations (0-140 μM), with 0 M (▲), middle concentration (●), and highest concentration (■). Slopes from LB plot trendlines were plotted against compound concentrations to generate K_i values as the x-intercept (insert graphs). Enzyme kinetic experiments were performed in triplicate and kinetic parameter values were averaged.