Research Paper Volume 17, Issue 3 pp 798—821

Figure 5. Older individual-derived OCs induced lower levels of cell expansion in NK cells. Osteoclasts (OCs) were generated as described in the Materials and Methods section. NK cells (0.5x10⁶ cells/ 2ml) were treated with a combination of IL-2 (1000 U/ml) and anti-CD16mAb (3μg/ml) for 18 hours before they were co-cultured with criss-cross OCs in the presence of probiotic bacteria sAJ2 (1:2:4: OCs:NK:sAJ2). NK cells were counted on days 6, 9, 12, 15, 18, 21, and 25, the day 0 counts were 0.5x10⁶ cells/2 ml, and 0.5x10⁶ cells/2 ml were cultured every 3 days (n=27, A). CD16+CD56+ NK cells, number of NK cells, and NK cells per one million of total expanding cells (B) and CD3+ T cells, number of T cells, and T cells per one million of total expanding cells (C) were determined on days 6, 9, 12, 15, 18, 21, and 25 (n=27). ****(p-value <0.0001), ***(p-value 0.0001-0.001).