Research Paper Volume 17, Issue 3 pp 610—629
Mitochondrial oxidative stress or decreased autophagy in osteoblast lineage cells is not sufficient to mimic the deleterious effects of aging on bone mechanoresponsiveness
Figure 2. Higher mechanical strain leads to periosteal woven bone formation in old mice. Left tibia of young (6 mo) and old (21 mo) C57BL/6J female mice were loaded with -10N and -8.5N, respectively, to achieve +1500με peak strain at the midshaft. (A) Micro-CT measurements at 5 mm proximal to the tibiofibular junction and at the midshaft of the right (non-loaded) and left (loaded) tibiae from young (n=13) and old (n=11) female mice. (B) Endosteal and periosteal perimeters at the tibia midshaft. (C) Representative photomicrographic cross-section images of the proximal tibia shaft. Scale bar, 200 μm. Bars represent mean ± SD. Data analyzed using two-way mixed ANOVA; p-values adjusted by Holm-Sidak’s test. Int: Interaction.