Research Paper Volume 17, Issue 3 pp 610—629
Mitochondrial oxidative stress or decreased autophagy in osteoblast lineage cells is not sufficient to mimic the deleterious effects of aging on bone mechanoresponsiveness
Figure 1. Aging reduces the periosteal osteogenic response to mechanical stimulation in female mice. (A) Schematic illustration of tibia axial compressive loading applied on mouse tibia with +1200με peak strain at the midshaft. (B) Experimental timeline describing the loading and fluorescent dye administration plan. (C) Micro-CT measurements at 5 mm proximal to the tibiofibular junction and at the midshaft of the right (non-loaded) and left (loaded) tibia from young (n=12) and old (n=9) female C57BL/6J mice. (D) Histomorphometric measurements at the periosteal tibial surface 5 mm proximal from the tibiofibular junction of young (n=6) and old (n=7) female mice, and (E) representative photomicrographic cross-section images. Scale bar, 200 μm. Bars represent mean ± SD. Data analyzed using two-way mixed ANOVA; p-values adjusted by Holm-Sidak’s test. Int: Interaction.