Research Paper Advance Articles

Figure 8. Honokiol ameliorates senescence–associated phenotypes and enhances skin barrier function. (A) Measurement of SA–β–gal positive cells (%). Senescent fibroblasts were treated with DMSO (0.01%) or honokiol (1 μM) for 12 days. ***P < 0.001, Student t–test. Mean ± S.D., N = 3. Scale bar 20 μm. (B) After 12 days of treatment with DMSO (0.01%) or honokiol (1 μM), autofluorescence was assessed in senescent fibroblasts by flow cytometry. The representative histogram of autofluorescence was shown. ***P < 0.001, Student t–test. Mean ± S.D., N = 3. (C) The expression levels of the SLIT2 in senescent fibroblasts were assessed after 12 days of treatment with DMSO (0.01%) or honokiol (1 μM). *P < 0.05, Student t–test. Mean ± S.D., N = 3. (D) The expression levels of the MMP1 in senescent fibroblasts were assessed after 12 days of treatment with DMSO (0.01%) or honokiol (1 μM). ***P < 0.001, Student t–test. Mean ± S.D., N = 3. (E) The expression levels of the CCL2 in senescent fibroblasts were assessed after 12 days of treatment with DMSO (0.01%) or honokiol (1 μM). ***P < 0.001, Student t–test. Mean ± S.D., N = 3. (F) The expression levels of the CCL5 in senescent fibroblasts were assessed after 12 days of treatment with DMSO (0.01%) or honokiol (1 μM). **P < 0.01, Student t–test. Mean ± S.D., N = 3. (G) The expression levels of the IL–6 in senescent fibroblasts were assessed after 12 days of treatment with DMSO (0.01%) or honokiol (1 μM). ***P < 0.001, Student t–test. Mean ± S.D., N = 3. (H) The expression levels of the IL–8 in senescent fibroblasts were assessed after 12 days of treatment with DMSO (0.01%) or honokiol (1 μM). ***P < 0.01, Student t–test. Mean ± S.D., N = 3.