Research Paper Advance Articles

Figure 1. Low-LET radiation-induced tumor development in Apc^1638N/+ mice is accompanied by an increased number of intestinal cells displaying senescence and SASP. (A) Intestinal-tumorigenesis at 150 days post-exposure (n=10 per group). Data presented as mean ± SEM, and * p<0.05, relative to control animals. (B) Representative H&E-stained micrographs of intestinal adenoma and carcinoma. (C) Quantification of adenoma and carcinoma as a percentage of total tumors. (D) Representative immunofluorescence micrographs of intestinal epithelium showing p16 (red) and IL6 (green) dual positive SASP cells. (E) Quantification of p16 and IL6 dual positive cells. Data presented as mean ± SEM, and * p<0.05, relative to control animals. (F). Representative immunofluorescence micrographs of intestinal epithelium showing p16 (red) and BCL-XL (green) dual positive senescent cells. (G). Quantification of p16 and BCL-XL dual positive cells. Data presented as mean ± SEM, and * p<0.05, relative to control animals.