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Figure 4. ABT-263 induced changes in the expression of some SASP factors. (A) Gene Set Enrichment Analysis (GSEA) enrichment plot for Reactome SASP gene set, which was not coordinately regulated with respect to ABT-263 treatment (p = 0.93). (B) GSEA enrichment plot showing that a set of 54 genes that are characterized as “increased” or “increased or no change” with SASP in Table 1 of Coppé et al. [13] are significantly coordinately upregulated with ABT-263 treatment (p < 0.001). (C) Heatmap of all genes described as in Coppé et al. [13]. Genes are presented in the same order and groups as in the source table; those with symbols in boldface are significantly regulated by ABT-263 (Wald FDR q < 0.05) in this experiment. Fold changes for downregulated genes are represented with their negative reciprocal (i.e., values of +2 and -2 correspond to two-fold up- or down-regulation by ABT-263, respectively). Variance-stabilizing-transformed expression values are z-score-normalized to a mean of zero and SD of 1 across all samples in each column, with blue, white and red indicating z scores of ≤ 2, 0, and ≥ 2, respectively.