Research Paper Volume 16, Issue 21 pp 13271—13287
Supplementation with nicotinamide limits accelerated aging in affected individuals with cockayne syndrome and restores antioxidant defenses
Figure 6. Level of nuclear POLG 1 upon supplementation with different doses of nicotinamide (NAM). (A) Representative images using a fluorescence microscope after treatment with NAM (0.5 and 1 mg/mL) for 24 h labeled with polg1 (Red) and nuclei with DAPI (blue) (the scale bar is 5 μm). (B) corresponding fluorescence intensity and QPCR results for the same gene, the p-values were calculated using unpaired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p<0,0001).