Research Paper Advance Articles

Figure 8. Mitochondrial dysfunction. Flow cytometry quantification of total reactive oxygen species using CM-H2DCFDA in MSC WT and MDPL ones. (A) Histogram representing the relative fluorescence intensity (FITCH-A), data are shown as mean ± SD of N = 3 biological replicates. Adjusted p-value was calculated using Student’s t-test. (B) Representative histogram plots in log scale (WT blue). Flow cytometry quantification of mitochondrial superoxide using MitoSOX Red in MSC WT and MDPL ones. (C) Histogram representing the relative fluorescence intensity (PE-A), data are shown as mean ± SD of N = 3 biological replicates. Adjusted p-value was calculated using Student’s t-test. (D) Representative histogram plots in log scale. (E) Histogram representing the quantification of red/green fluorescence intensity ratio, data are shown as mean ± SD of N = 3 biological replicates. Adjusted p-value was calculated using Student’s t-test. (F) Representative dot plots in log scale of JC1 stained MSC WT and MDPL cells. The WT data are derived from the mean of the values obtained from the two healthy control samples; ^*p < 0.1, ^**p < 0.01.