Research Paper Volume 16, Issue 21 pp 13252—13270

Figure 2. Characterization of extracellular vesicles isolated from ASCs. (A) Representative picture of one single EV by cryo-transmission electron microscopy (scale bar: 100 nm). (B) Size distribution of EVs (one representative sample). (C) Number of particles produced by 10⁶ ASCs per day, mean size and particle concentration after isolation (n = 7). (D) Quantity of total proteins (left panel) and total RNA (right panel) contained per particle (n = 3). (E) Expression profile of tetraspanin markers on the surface of EVs by nanofcm analysis.