Research Paper Volume 16, Issue 21 pp 13356—13370
LncRNA DANCR suppresses acute myocardial infarction in mice via mediating p-RXRA/TRAF2/NIK/IKK/NF-κB signaling pathway
Figure 8. Silencing DANCR induced cardiomyocyte apoptosis by activating the NIK/IKK/NF-κB pathway. H9C2 cells were transfected with DANCR shRNA (sh-DANCR) and control (sh-NC), and then treated with OGD and/or B022 (an inhibitor of NIK). (A) The expression of DANCR was detected with RT-qPCR. (B–D) The protein expression of p-RXRA and TRAF2 was detected with Western blotting. (E, F) The activity of NIK and IKK was measured by ELISA. (G–I) The protein expression of p-Ik-Bα and p-p65 was detected with Western blotting. (J) Cell viability was measured by using CCK-8. (K) The activity of caspase-3 was assayed by ELISA. (L, M) Cell apoptosis was assessed by using Flow cytometry. *P < 0.05, **P < 0.01.