Research Paper Volume 16, Issue 21 pp 13356—13370
LncRNA DANCR suppresses acute myocardial infarction in mice via mediating p-RXRA/TRAF2/NIK/IKK/NF-κB signaling pathway
Figure 7. Overexpression of TRAF2 reversed the protective effect of Bex on cardiomyocytes. H9C2 cells were transfected with TRAF2 overexpression vector (pc-TRAF2) or control (vector), and then treated with OGD and/or Bex. (A–C) The protein expression of p-RXRA and TRAF2 was detected with Western blotting. (D) Cell viability was measured by using CCK-8. (E, F) Cell apoptosis was assessed by using Flow cytometry. (G) The activity of caspase-3 was assayed by ELISA. (H–J) Western blotting was used to detect the protein levels of Bcl-2 and Bax. *P < 0.05, **P < 0.01.