Figure 3.Effects of DANCR overexpression on the behavior of cardiomyocytes. (A) The expression of DANCR in H9C2 cells treated with OGD for 0, 2, 6, 12 and 24 h. H9C2 cells were transfected with DANCR overexpression vector (pc-DANCR) or control (vector), and then treated with OGD for 24 h. (B) The expression of DANCR was detected with RT-qPCR. (C) Cell viability was measured by using CCK-8. (D, E) Cell apoptosis was assessed by using Flow cytometry. (F) The activity of caspase-3 was assayed by ELISA. (G, H) Western blotting was used to detect the protein levels of Bcl-2 and Bax. *P < 0.05, **P < 0.01.
