Research Paper Volume 16, Issue 21 pp 13356—13370
LncRNA DANCR suppresses acute myocardial infarction in mice via mediating p-RXRA/TRAF2/NIK/IKK/NF-κB signaling pathway
Figure 3. Effects of DANCR overexpression on the behavior of cardiomyocytes. (A) The expression of DANCR in H9C2 cells treated with OGD for 0, 2, 6, 12 and 24 h. H9C2 cells were transfected with DANCR overexpression vector (pc-DANCR) or control (vector), and then treated with OGD for 24 h. (B) The expression of DANCR was detected with RT-qPCR. (C) Cell viability was measured by using CCK-8. (D, E) Cell apoptosis was assessed by using Flow cytometry. (F) The activity of caspase-3 was assayed by ELISA. (G, H) Western blotting was used to detect the protein levels of Bcl-2 and Bax. *P < 0.05, **P < 0.01.