Research Paper Volume 16, Issue 19 pp 12928—12951

Figure 3. GATA2 directly promotes the transcriptional activity of Abca1 and Pacsin1. GO enrichment analysis was done for upregulated/downregulated differentially expressed genes and different GO pathways and the related DEGs were connected with different colored bars (A). Schematic drawing of the mouse Abca1/Pacsin1 promoter region with potential GATA2 DNA binding sites (black boxes) and that of the pGL6-Abca1/Pacsin1-Luc construct containing the Abca1/Pacsin1 promoter region (B). They were co-transfected in mouse aortic endothelial cells (MAECs) and relative luciferase activity was measured. The mutant plasmid was then constructed and co-transfected, the relative luciferase activity was measured as well (C). ^*P < 0.05 vs. Vector and #P< 0.05 vs. Gata2-WT.