Research Paper Volume 16, Issue 19 pp 12909—12927

Figure 2. Knockdown of MIAT suppresses the myofibroblastic properties. (A–D) Primary fBMFs (obtained from two patients with OSF; fBMFs−1 and −2) were transfected with lentiviruses expressing non-targeting ShRNA (Sh-Luc.) and Sh-MIAT (Sh-MIAT−1 and −2). The MIAT knockdown efficiency was assessed using qRT-PCR analysis (A). The cells (fBMFs−1 and −2) were then cultured in collagen gel for additional 48 hours, and the gel area after cell contraction was measured (B). Cells (fBMFs−1 and −2) were cultured in Transwell system for an additional 24 hours, and their migration ability was quantified (C). Data are presented as mean ± SD (n=3); *p < 0.05 vs. Sh-Luc. (A–C). Confluent monolayers of fBMFs−2 were scratched and cultured for 48 hours, and the wound closure was assessed. Scale bar, 50 μm (D).